SPE solid phase extraction device (SPE solid phase extraction device) is a kind of widely used and popular sample pretreatment equipment. It uses solid adsorbent to selectively adsorb the target compounds in the sample, separate them from the sample matrix and interfering substances, and then elute them with eluent; or use solid adsorbent to adsorb the target compounds in the sample, separate them from the interfering substances, and then wash them through washing The aim of separation and enrichment of target compounds can be achieved by washing out the target compounds. SPE solid phase extraction device is mainly used to enrich and purify samples, and when some special instruments have special requirements for sample solvent, it can be transformed through SPE column.
Solid phase extraction (SPE) is a sample pretreatment technology developed since the mid-1980s. It is developed by the combination of liquid-solid extraction and liquid chromatography. It is mainly used for separation, purification and enrichment of samples. The main purpose is to reduce matrix interference and improve detection sensitivity.
SPE technology is based on the theory of liquid-solid chromatography, which uses selective adsorption and selective elution to enrich, separate and purify samples. It is a physical extraction process including liquid phase and solid phase; it can also be regarded as a simple chromatographic process approximately.
SPE is a separation principle of liquid chromatography based on selective adsorption and selective elution. The commonly used method is to make the liquid sample solution pass through the adsorbent, retain the substance to be measured, then select the solvent with appropriate strength to wash off the impurities, and then use a small amount of solvent to quickly elute the tested material, so as to achieve the purpose of rapid separation, purification and concentration. It can also selectively adsorb interfering impurities and let the measured substances flow out; or adsorb impurities and measured substances at the same time, and then use appropriate solvents to selectively elute the measured substances.
The extractant of solid phase extraction method is solid, and its working principle is based on that the components to be measured and the coexisting interfering components in water sample have different force on the solid phase extraction agent, so that they can be separated from each other. Solid phase extraction (SPE) is a special filler containing C18 or C8, nitrile, amino groups and other groups.
According to the filler or solvent composition used in the process of solid phase extraction, the general oil-free vacuum pump can be used for the vacuum pump equipped with solid phase extraction instrument. The oil-free vacuum pump has the characteristics of maintenance free, oil-free design, and will not cause pollution.
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